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KMID : 0364820080440010069
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Korean Journal of Microbiology
2008 Volume.44 No. 1 p.69 ~ p.73
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Characterization of β-1,4-Glucanase Activity of Bacillus licheniformis B1 in Chungkookjang
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Hwang Jae-Sung
Yoo Hyung-Jae
Kim Han-Bok
Kim Sung-Jo
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Abstract
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Fermented soybeans contain microorganisms, diverse enzymes, and bioactive compounds. Few studies on cellulase
in Chungkookjang exist. Oligosaccharides play diverse roles of bioactivity. Through Congo red test and activity staining, it was confirmed that the enzyme solution contained cellulase. Optimal pH and temperature of the cellulase produced by Bacillus licheniformis B1 were 10 and 40oC, respectively. Through TLC analysis, it was demonstrated that the enzyme solution degraded carboxymethyl cellulose (CMC), whose main products contained dimer and trimer oligosaccharides. Cellulase activity of barley-Chungkookjnag fermented by the strain increased, compared with that of Chungkookjang. The cellulase was found to be a β-1,4-glucanase through the analysis of the cloned gene, showing polymorphism at 32 amino acid sites in the coding range of amino acid 10 and 460.
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KEYWORD
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Bacillus licheniformis, β-1, 4-glucanase, cellulose, Chungkookjang
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